Synthesized Pheophorbide a‐mediated photodynamic therapy induced apoptosis and autophagy in human oral squamous carcinoma cells
Identifieur interne : 001158 ( Main/Exploration ); précédent : 001157; suivant : 001159Synthesized Pheophorbide a‐mediated photodynamic therapy induced apoptosis and autophagy in human oral squamous carcinoma cells
Auteurs : Mee Young Ahn [Corée du Sud] ; Hyo-Eun Yoon [Corée du Sud] ; Seong-Min Kwon [Corée du Sud] ; Jun Lee [Corée du Sud] ; Seung-Ki Min [Corée du Sud] ; Yong-Chul Kim [Corée du Sud] ; Sang-Gun Ahn [Corée du Sud] ; Jung-Hoon Yoon [Corée du Sud]Source :
- Journal of Oral Pathology & Medicine [ 0904-2512 ] ; 2013-01.
English descriptors
- Teeft :
- Acridine, Actin, Actin levels, Annexin, Annexin apoptotic cells, Apoptosis, Apoptotic, Apoptotic cell death, Apoptotic cells, Assay, Autophagy, Autophagy inhibition, Autophagy inhibitor, Beckman coulter, Carcinoma, Cell death, Cell proliferation, Cell survival, Cell viability, Control cells, Culture medium, Cytometry analysis, Daejeon, Exclusion assay, Hmgb1, Human oscc cells, Independent experiments, Laser, Mitochondrial membrane, Molecular mechanisms, Molecular probes, Neck cancer, Necrosis, Necrotic, Necrotic cell death, Necrotic cells, Oral pathol, Oscc, Oscc cells, Parp, Parp cleavage, Pathol, Pathway, Photodynamic, Photodynamic therapy, Protein levels, Reactive oxygen species, Representative bands, Untreated control, Uorescence, Uorescence microscope, Uorescence microscopy, Viable cells, Western blot analysis, Wonkwang university.
Abstract
Background: Pheophorbide a (Pa) is a chlorine‐based photosensitizer derived from an ethnopharmacological herb, and our group recently synthesized Pa by the removal of a magnesium ion and a phytyl group from chlorophyll‐a. In this study, the effect of photodynamic therapy (PDT) with synthesized Pa was examined in a human oral squamous cell carcinoma (OSCC) cells. Methods: Cells were treated with PDT with Pa, and reactive oxygen species (ROS) and mitochondrial membrane potential [ΔΨ (m)] were examined. Apoptosis was measured using annexin V staining and immunoblot. Autophagy was characterized by the increase in LC3B‐II and the formation of autophagosome and acidic vesicular organelles (AVOs). Results: Pa‐PDT inhibited the proliferation of OSCC cells in a dose‐dependent manner. Pa‐PDT increased the number of apoptotic cells by inactivating ERK pathway. Pa‐PDT also induced autophagy in OSCC cells evidenced by the increased levels of LC3 type II expression and the accumulation of AVOs. The inhibition of autophagy enhanced Pa‐PDT‐mediated cytotoxicity through an increase in necrosis. Conclusions: These results suggest that synthesized Pa‐PDT exerts anti‐tumor effects by inducing apoptosis and autophagy and provide novel evidence that Pa‐PDT induces autophagy, and autophagy inhibition enhances Pa‐PDT‐mediated necrosis in OSCC cells.
Url:
DOI: 10.1111/j.1600-0714.2012.01187.x
Affiliations:
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coulter</term><term>Carcinoma</term><term>Cell death</term><term>Cell proliferation</term><term>Cell survival</term><term>Cell viability</term><term>Control cells</term><term>Culture medium</term><term>Cytometry analysis</term><term>Daejeon</term><term>Exclusion assay</term><term>Hmgb1</term><term>Human oscc cells</term><term>Independent experiments</term><term>Laser</term><term>Mitochondrial membrane</term><term>Molecular mechanisms</term><term>Molecular probes</term><term>Neck cancer</term><term>Necrosis</term><term>Necrotic</term><term>Necrotic cell death</term><term>Necrotic cells</term><term>Oral pathol</term><term>Oscc</term><term>Oscc cells</term><term>Parp</term><term>Parp cleavage</term><term>Pathol</term><term>Pathway</term><term>Photodynamic</term><term>Photodynamic therapy</term><term>Protein levels</term><term>Reactive oxygen species</term><term>Representative bands</term><term>Untreated control</term><term>Uorescence</term><term>Uorescence microscope</term><term>Uorescence microscopy</term><term>Viable cells</term><term>Western blot analysis</term><term>Wonkwang university</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Pheophorbide a (Pa) is a chlorine‐based photosensitizer derived from an ethnopharmacological herb, and our group recently synthesized Pa by the removal of a magnesium ion and a phytyl group from chlorophyll‐a. In this study, the effect of photodynamic therapy (PDT) with synthesized Pa was examined in a human oral squamous cell carcinoma (OSCC) cells. Methods: Cells were treated with PDT with Pa, and reactive oxygen species (ROS) and mitochondrial membrane potential [ΔΨ (m)] were examined. Apoptosis was measured using annexin V staining and immunoblot. Autophagy was characterized by the increase in LC3B‐II and the formation of autophagosome and acidic vesicular organelles (AVOs). Results: Pa‐PDT inhibited the proliferation of OSCC cells in a dose‐dependent manner. Pa‐PDT increased the number of apoptotic cells by inactivating ERK pathway. Pa‐PDT also induced autophagy in OSCC cells evidenced by the increased levels of LC3 type II expression and the accumulation of AVOs. The inhibition of autophagy enhanced Pa‐PDT‐mediated cytotoxicity through an increase in necrosis. Conclusions: These results suggest that synthesized Pa‐PDT exerts anti‐tumor effects by inducing apoptosis and autophagy and provide novel evidence that Pa‐PDT induces autophagy, and autophagy inhibition enhances Pa‐PDT‐mediated necrosis in OSCC cells.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Ahn, Mee Young" sort="Ahn, Mee Young" uniqKey="Ahn M" first="Mee Young" last="Ahn">Mee Young Ahn</name></noRegion><name sortKey="Ahn, Sang Un" sort="Ahn, Sang Un" uniqKey="Ahn S" first="Sang-Gun" last="Ahn">Sang-Gun Ahn</name><name sortKey="Kim, Yong Hul" sort="Kim, Yong Hul" uniqKey="Kim Y" first="Yong-Chul" last="Kim">Yong-Chul Kim</name><name sortKey="Kwon, Seong In" sort="Kwon, Seong In" uniqKey="Kwon S" first="Seong-Min" last="Kwon">Seong-Min Kwon</name><name sortKey="Lee, Jun" sort="Lee, Jun" uniqKey="Lee J" first="Jun" last="Lee">Jun Lee</name><name sortKey="Min, Seung I" sort="Min, Seung I" uniqKey="Min S" first="Seung-Ki" last="Min">Seung-Ki Min</name><name sortKey="Yoon, Hyo Un" sort="Yoon, Hyo Un" uniqKey="Yoon H" first="Hyo-Eun" last="Yoon">Hyo-Eun Yoon</name><name sortKey="Yoon, Jung Oon" sort="Yoon, Jung Oon" uniqKey="Yoon J" first="Jung-Hoon" last="Yoon">Jung-Hoon Yoon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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